Antibody-Based Technologies => Antibodies Review => Antibody B => Topic started by: maya on December 23, 2006, 10:10:12 AM

Title: Bcl1/cyclin D1 protocol
Post by: maya on December 23, 2006, 10:10:12 AM
I've started using the ventana system and i can't figure what is the suitable protocol for BCl1/cyclin D1 mAb. I've tried amplifying and using lower Ab dilution but the staining is still very weak.
Does anybody have any sugestions?
thanks
Maya.
Title: Re: Bcl1/cyclin D1 protocol
Post by: dabaicai on March 21, 2009, 07:54:17 PM
I have got good results from using CyclinD1 antibody (mouse, BD Pharmingen, 1:200) on 4% PFA fixed, paraffin-embedded embryonic brain tissue sections, with regular IHC plus citrate buffer retrieval.
Title: Re: Bcl1/cyclin D1 protocol
Post by: gula on March 22, 2009, 04:11:50 AM
We perform Cyclin D1 (SP4, Neomarker) with CC1 mild, 1:25, 24 min, 37C on the Benchmark XT.
gula