Antibody-Based Technologies => Antibodies Review => Antibody G => Topic started by: ImmunoNYC on February 09, 2007, 12:36:14 AM

Title: GFAP in mouse brain
Post by: ImmunoNYC on February 09, 2007, 12:36:14 AM
Two questions:

(1) What is your most recommended antibody for this purpose. I have access to paraffin and frozen (frozen are preferred).
(2) What sort of staining pattern can I expect as far as frequency of positive events.

I have used the DAKO rabbit pAb with success but want to confirm my results or whether another antibody is generally preferred for mouse tissue.
Title: Re: GFAP in mouse brain
Post by: excalibur on February 09, 2007, 10:47:52 AM
GFAP is one of the first antibodies I used years ago. It is great because it works on frozen and paraffin with no AR. Any major company will have GFAP. Most are less expensive than DAKO. ;)
Title: Re: GFAP in mouse brain
Post by: ImmunoNYC on February 10, 2007, 12:29:53 AM
Thanks Paula, have you use any particular one on mouse brain?
Title: Re: GFAP in mouse brain
Post by: hardrob on February 14, 2007, 08:03:35 AM
I've used GFAP( concentrated- Dilution 1:200/2 hours) from Biogenex many times on mouse brain. Worked perfect.


Title: Re: GFAP in mouse brain
Post by: Joannak on February 15, 2007, 04:51:16 PM
Hi,

I'm using GFAP antibody from Chemicon (MAB360) and I can relay recommend it. I used it only on frozen section and it was working perfectly. Unfortunately this one is not anti-mouse, so if you want to stain mouse's sections you will have to use M.O.M kit. But I tried this and it is working as well.

Greets,

Joanna
Title: Re: GFAP in mouse brain
Post by: ImmunoNYC on February 16, 2007, 12:23:48 AM
Thank you all for your suggestions. Can someone post a photo of what expected GFAP pattern is like in the brain and also eye? Thanks!