General Research Topics => Biochemistry => Topic started by: raoqing on July 12, 2007, 02:15:18 AM

Title: intracellular juxtamembrane protein staining
Post by: raoqing on July 12, 2007, 02:15:18 AM
I want to stain a protein existin in intracellular juxtamembrane.Do I need penetrate the cell membrane before adding antibody.If need, how penetrate it?
Thanks 
Title: Re: intracellular juxtamembrane protein staining
Post by: ImmunoNYC on November 14, 2008, 01:36:46 PM
I assume you are staining cells grown on slides or something similar??? If your antibody is targeted towards an antigen expressed on the inside of the cell (for example the c-terminus of a protein that spans the lipid bilayer) then you would need to permeabilize - especially if your antibody is a monoclonal antibody. Do you know anything about your antibody? Is it polyclonal, monoclonal? If in doubt why not try it on positive control samples with and without permeabilization.