General Research Topics => Microbiology => Topic started by: helfi on January 16, 2008, 01:19:49 AM

Title: positive control for HCV
Post by: helfi on January 16, 2008, 01:19:49 AM
Dear all,

I'm working on experiments on Hepatitis C Virus at the moment. I use formalin fixed parraffin  embedded slide from surgical specimen. At first I tried ISH using RNAprobe from MicroProbe. I got a result which at first I thought was positive, but it was just a debris. The method itself was modification here and there from an IHC kit.
I tried several time and came up with no result.
Now we switch to use IHC. I have to work on my own protocols also for this experiment. I am a newcomer in research works, therefore I'm not really sure whether my methods is correct or not. I'm also the first person to do these methods in our lab.
I was wondering if anyone can help me, where can I get commercially available positive control slide for ISH and IHC on Hep C Virus?
I previously tried ISH kit for EB Virus and found that its positive control slide was really helpful.

million thanks,

Helfi
Title: Re: positive control for HCV
Post by: nnguyen on February 11, 2008, 08:53:34 PM
I'm not too sure if they're are commercially available positive control slides for HCV.

Your best bet is to find a subject with a known high viral load.

BTW which epitope of the HCV is your antibody directed to?

Regards,
Nam