Methods and Techniques Discussion => Western Blotting (WB) => Topic started by: Welly S on May 07, 2008, 12:01:53 AM

Title: HIF-1a
Post by: Welly S on May 07, 2008, 12:01:53 AM
Hi there  ;D,

 it is the first time for me to perform WB using HIF-1a antibody. The HIF-1a protein expression level should occurs at 120kd, however, the expression in my study always occurred between 70-90 kd, even the method repeated many times, the result always same.  Does any one have a comment or suggestion on this matter? Your assistance is highly appreciated. ;)