Methods and Techniques Discussion => Apoptosis or Cell Death => Topic started by: voland on October 04, 2008, 08:33:46 AM

Title: Silver stainings?
Post by: voland on October 04, 2008, 08:33:46 AM
Hi,

Has anybody any experience with silver stainings on brain tissue? Is it better tha FluoroJade to show neurodegeneration/cell death?
Title: Re: Silver stainings?
Post by: richard03 on October 04, 2008, 05:49:47 PM
Olmos's amino cupric sliver staining method is commonly used for neurodegeneration. But FluoroJade is probably easier method.

A application for sliver method.

Neuronal Degeneration in Canine Narcolepsy
http://www.npi.ucla.edu/sleepresearch/CanineArticle/CanineArticle.htm

A most recent paper about Olmos sliver method.

A modified silver technique (de Olmos stain) for assessment of neuronal and axonal degeneration.
http://www.ncbi.nlm.nih.gov/pubmed/18309923