General Research Topics => Cell Biology => Topic started by: science on October 13, 2008, 04:26:09 PM

Title: Organelle Fixation Protocols
Post by: science on October 13, 2008, 04:26:09 PM
Is there a chart that describes what fixation methods work best for various organelles? What works well for lysosomes?
Title: Re: Organelle Fixation Protocols
Post by: richard03 on October 13, 2008, 11:04:10 PM
In general, formalin or formaldehyde is the best fixative for preserving tissue and cell morphology so it should work well for various organelles.
Title: Re: Organelle Fixation Protocols
Post by: science on October 14, 2008, 12:18:35 AM
where can I find a good formalin fixation protocol?
Title: Re: Organelle Fixation Protocols
Post by: richard03 on October 14, 2008, 07:04:41 AM
You can buy a bottle of formaldehyde and dilute 1:10 with water. Here is a protocol list for your reference.

http://www.ihcworld.com/_protocols/histology/fixatives.htm
Title: Re: Organelle Fixation Protocols
Post by: monia on February 28, 2009, 01:08:13 PM
Effect of fixation protocols on muscle preservation and ... relative organelle
volumes is fairly insensitive to variations in preparative ...
Title: Re: Organelle Fixation Protocols
Post by: ImmunoNYC on March 06, 2009, 11:39:54 PM
Ruchard why don't you suggest to dilute in PBS which is what I would do? Just curious ...
Title: Re: Organelle Fixation Protocols
Post by: richard03 on March 07, 2009, 02:32:08 PM
That was just protocol I have been using. But I agree that Buffered Formalin should work better than Un-buffered formalin, especially in preserving ultrastructure.

Ruchard why don't you suggest to dilute in PBS which is what I would do? Just curious ...