Antibody-Based Technologies => Antibodies Review => Antibody M => Topic started by: Lou on August 31, 2009, 12:21:55 PM

Title: MAC387
Post by: Lou on August 31, 2009, 12:21:55 PM
Looking for a good macrophage antibody (who does not!?), I was proposed to use MAC387. Has anybody already use it? I read that it also stains granulocytes, can it still be considered as a good macrophage marker?
Thank you so much for your remark
L.
Title: Re: MAC387
Post by: richard03 on August 31, 2009, 10:58:16 PM
CD163, CD68 and F4/80 are commonly used macrophage markers. Read more..

http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm
Title: Re: MAC387
Post by: Lou on September 04, 2009, 05:37:02 AM
I forgot to mention that I work in humans so F4/80 i can forget and its human form, EMR1 if I remember well is not a good macrophage marker acoording to several publications.
CD163 is the mannose receptor so macrophages oriented towards the "M2" anti-inflammatory way and CD68 is currently discussed to be slightly "M1" oriented...
ALthough CD68 might be the best, I was just asking if someone got an idea on another good "general" macrophages marker...