Antibody-Based Technologies => Cell Markers Review => Topic started by: babayao on October 08, 2009, 10:58:41 AM

Title: Podocyte markers
Post by: babayao on October 08, 2009, 10:58:41 AM
I am trying to mark podocyte with either WT-1 or GLEPP1 by IHC or IF in rat kidney.
Is there anyone deal with these two Abs before? Could we share experience?

When I try WT-1 from santa cruz, I see a lot of background on tubullar cells but not in glomerulus where it should be positive.

For the GLEPP1, I am not sure which Ab is better. According to the literature, the good one is using a monoclonal antibody produced by themselves. Is that means all commercial Abs are not good? or I should try one?

Any suggestions are welcome!

Thanks in advance!