Methods and Techniques Discussion => Image Analysis => Topic started by: funk.106 on April 21, 2010, 04:57:24 PM

Title: direct versus diffuse colocalization
Post by: funk.106 on April 21, 2010, 04:57:24 PM
Hi everyone,
I have a question regarding the best way to express my IHC results. Basically, I am using one ab to mark a specific intracellular lesion and then a panel of abs to try to learn about the lesion in a process, so each ab represents a different point in maturation in the process continuum. So when I stain my tissue, I see different kinds of colocalization depending on which antibody I am l looking at--I have grouped it into either "direct" colocalization, which there is either pixel overlap or one is encircling the other, or "indirect" colocalization, in which case the protein seems to be diffusely upregulated in the cell but not necessarily direcly colocalized with the subcellular lesion. I am trying to find other examples of papers that have found similar types of results that I could model my analysis and expression after but thus far have not had much luck. If anyone has any suggestions, I would greatly appreciate it!!