Methods and Techniques Discussion => Western Blotting (WB) => Topic started by: funk.106 on July 21, 2010, 03:55:35 PM

Title: p62 western blot
Post by: funk.106 on July 21, 2010, 03:55:35 PM
Hi everyone,
I recently did a western blot on Alzheimer's disease brain and probed for the autophagic protein marker p62--mainly just checking for antibody specificity, so no experimental stuff yet. This is my first time blotting with this antibody, though the IHC works really nicely, and I didn't quite get the bands that I expected. I don't know if it's just a loser antibody that gives a lot of non-specific binding or its just that its a polyubiquitin binding protein, so it has a lot of interacting partners--ie lots of bands. I'm hoping that someone out there has had experience working with this protein and can maybe give some insight as to what these extra bands are.
Thanks for any help!