Methods and Techniques Discussion => Immunocytochemistry (ICC) => Topic started by: cincanada on November 03, 2010, 01:48:45 PM

Title: New to me
Post by: cincanada on November 03, 2010, 01:48:45 PM
So I have never done this type of immuno before.  We want to stain cells and blood vessels.  Do I need to fix these?  I have worked strictly with fixed tissue in the past (PFA/Sucrose).

Please advise the best way to preserve these and follow through with Immuno.

C
Title: Re: New to me
Post by: MT Scientist on November 03, 2010, 02:08:47 PM
Need more information.....
Title: Re: New to me
Post by: Cardio on November 03, 2010, 04:51:32 PM
So I have never done this type of immuno before.  We want to stain cells and blood vessels.  Do I need to fix these?  I have worked strictly with fixed tissue in the past (PFA/Sucrose).

Please advise the best way to preserve these and follow through with Immuno.

C

Fixation normally depends on your antibody. You can find out what fixation to use on the antibody datasheet. Normally they have a protocol section. IF they don't then call the company for more information.