Methods and Techniques Discussion => Antigen Retrieval => Topic started by: mardinly on August 18, 2003, 01:25:17 PM

Title: Antigen Retreival
Post by: mardinly on August 18, 2003, 01:25:17 PM
Hello all,

I optimized my antigen retrieval procedure using a standard citrate buffer from vector in a hot water bath for 20 minutes, followed by 20 minutes cooling.  However, when I started staining patient samples, I noticed that due to the higher fat content, these samples had a nasty tendency to boil off the slides.  I thus had to drastically reduce the boil of the hot water bath.  I am now faced with the alternative of having a completely ineffective antigen retrieval step or having the sample slide off the slides.  Does anybody know an antigen retreival method that does not involve so much heat?  

Many thanks,
Alan
Title: Antigen Retreival
Post by: mardinly on August 18, 2003, 03:44:24 PM
after doing a little looking around, the Formic Acid method looks appealing.  Has anybody tried it? If so, how does it compare with water bath methods? Thanks.

Alan
Title: Antigen Retreival
Post by: Marianne on August 26, 2003, 09:01:02 AM
Hi Alan

Try using coated slides (e.g Superfrost+)

Marianne