Antibody-Based Technologies => Cell Markers Review => Topic started by: fransisca on February 11, 2011, 07:14:44 PM

Title: F4/80 staining
Post by: fransisca on February 11, 2011, 07:14:44 PM
Dear All,
I'm going to do F4/80 staining on mouse brains that have been fixed in formalin at 4C for more than 24 hours (~40 hours). I know it's not supposed to be that long. However, since I don't have any more mice, do you have any advice on how to make these tissues work for F4/80 immunostaining? Any special recipe for permeabilization of this overly-fixed tissue? Thanks so much..

-Fransisca-
Title: Re: F4/80 staining
Post by: richard03 on February 13, 2011, 02:03:23 PM
40 hour fixation is not too long. With antigen retrieval, you will be able to make it to work. Refer to this protocol for more information.

http://www.ihcworld.com/_protocols/antibody_protocols/f4.80_caltag.htm
Title: Re: F4/80 staining
Post by: inmuno on October 31, 2013, 12:20:13 PM
Try with proteinase k 5 minutes and after that a citrate buffer bath of five minutes.It works for my F4/80 antibody (Serotec)

hope this helps