Methods and Techniques Discussion => Image Analysis => Topic started by: derm81 on February 15, 2011, 10:45:53 AM

Title: IOD in Imagepro plus?
Post by: derm81 on February 15, 2011, 10:45:53 AM
 I am trying to use the Image pro plus software to quantify my IHC images. I have come across many publications that have used Integrated optic density (IOD) as a parameter for quantification. I was wondering what IOD is exactly  ??? and what does it signify. If any could help me understand this concept please :).
Title: Re: IOD in Imagepro plus?
Post by: MT Scientist on February 15, 2011, 12:37:48 PM
From the ImagePro website:
"Integrated Optical Density (IOD): Reports the average intensity/density of each object. This value will be expressed in terms of the current intensity/density mode and calibration."

The best explanation I have found: IOD = area x average density

More info can be found at: http://www.mediacy.com/ and http://www.mediacy.com/index.aspx?page=ProductNotes

I have used the IOD in past to quantify relative fluorescence in my slides.
Title: Re: IOD in Imagepro plus?
Post by: MT Scientist on February 15, 2011, 12:42:22 PM
From the ImagePro website:
"Integrated Optical Density (IOD): Reports the average intensity/density of each object. This value will be expressed in terms of the current intensity/density mode and calibration."

The best explanation I have found: IOD = area x average density (intensity?)

More info can be found at: http://www.mediacy.com/ and http://www.mediacy.com/index.aspx?page=ProductNotes

I have used the IOD in past to quantify relative fluorescence in my slides.
Title: Re: IOD in Imagepro plus?
Post by: derm81 on February 23, 2011, 04:23:22 PM
From the ImagePro website:
"Integrated Optical Density (IOD): Reports the average intensity/density of each object. This value will be expressed in terms of the current intensity/density mode and calibration."

The best explanation I have found: IOD = area x average density

More info can be found at: http://www.mediacy.com/ and http://www.mediacy.com/index.aspx?page=ProductNotes

I have used the IOD in past to quantify relative fluorescence in my slides.

thanks for your reply. You have mentioned in your post that you have used IOD previously, so do you think its an appropriate parameter for comparing staining between different sections, for eg. to assess a treatment effect from the staining pattern by comparing staining pre and post treatment
Title: Re: IOD in Imagepro plus?
Post by: MT Scientist on February 23, 2011, 05:24:13 PM
Depends. 
It worked for us, but may not work for you.  Unfortunately only you will be able to determine whether or not it applies to your particular study.  It may also depend on the particular stain you are doing.