Methods and Techniques Discussion => Enzyme Linked ImmunoSorbentAssay (ELISA) => Topic started by: Grazi on January 20, 2004, 06:00:19 AM

Title: ELISA
Post by: Grazi on January 20, 2004, 06:00:19 AM
Hi everybody!!!   :D

I am working with ELISA method which use Strepdavidin-HRP. Last week the chromogen(TMB) finished.... :(  but I still need to keep working with ELISA...
So, I would like to know if somebody has a protocol which teaches how to make TMB!
Maybe, somebody has another idea to improve an adaptation to this protocol....maybe another chromogen that works with  Strepdavidin-HRP...

Any help will be apreciated!!!
Thanks a lot!!!
Grazi
Title: Re: ELISA
Post by: richard03 on September 27, 2007, 10:48:34 PM
TMB protocol

http://www.ihcworld.com/_protocols/chromogen_substrates/POD_TMB_blue.htm
Title: Re: ELISA
Post by: VTimberlake on September 18, 2015, 01:39:37 AM
Have you tried sandwich Elisa before?