Methods and Techniques Discussion => Apoptosis or Cell Death => Topic started by: rgodar on March 15, 2011, 03:22:11 PM

Title: warped slide
Post by: rgodar on March 15, 2011, 03:22:11 PM
I just tried TUNEL staining on my own for the first time. I previously got it to work perfectly, partially assisted by my PI. This time there was no staining except along one edge of the slide. Also, after I removed the slide from the humidified chamber, it was warped. Is it possible this would cause the stain to not set properly on the cells? I did have difficulty getting the cover slip to stay because of the warping. Has anyone had this problem or know how to fix it? Thanks.
Title: Re: warped slide
Post by: excalibur on March 15, 2011, 03:25:21 PM
A glass slide will not warp. Did you use a plastic slide?