Methods and Techniques Discussion => Western Blotting (WB) => Topic started by: MGoodfellow on June 27, 2012, 11:47:32 AM

Title: Ladder Ghost Bands
Post by: MGoodfellow on June 27, 2012, 11:47:32 AM
I have been unable to get real bands for my ladder- just ghost bands. I use 10 ul (the recommended volume) in 4-20% mini gels and transfer for 1 hr at 100V. I have tried decreasing my secondary concentration, increasing blocking time/concentration, and decreasing ECL substrate incubation time- still no ladder. What else can I try? I'm new to WB so any suggestions would be appreciated.
Title: Re: Ladder Ghost Bands
Post by: Sindy on January 17, 2013, 12:11:34 AM
 :)Hi there. I am not sure.. are you using a strep tag ladder ?