Methods and Techniques Discussion => Western Blotting (WB) => Topic started by: MMehdi on July 12, 2012, 11:05:15 AM

Title: Concentrate the proteins or precipitate them?
Post by: MMehdi on July 12, 2012, 11:05:15 AM
Hi,

Either due to an error in the Bradford or too much dilution with water, my diluted samples (with sample buffer) of retinas contain a variable amount of protein, checked by beta-actin. The maximum sample size is 700 micro liters. I want to re-assay proteins in my samples. Bradford seems to be incompatible with Lamelli-sample buffer detergents. I'm looking for a protein assay which is compatible with sample buffer and other salts of Protein extraction buffer. Please do not suggest commercial kits.
Thanks