General Research Topics => Cell Biology => Topic started by: ic2133 on December 17, 2012, 12:02:26 PM

Title: Cell culture maintenance and confluency
Post by: ic2133 on December 17, 2012, 12:02:26 PM
Hi,

I am growing HEK 293 cells. I know you're supposed to passage them at 70%, but I let them grow to 100% by accident.  Can I still pass them and maintain them, as long as my experiments are done on 70% confluent cells? How bad is it that it got to 100%?

Thank you!
Title: Re: Cell culture maintenance and confluency
Post by: AArwen on January 16, 2013, 05:43:03 PM
In my experience is really not a problem if you passage them at 100%, especially with HEK cells. When you passage them just keep in mind to dilute them a bit more since you've more cells than usual.
Title: Re: Cell culture maintenance and confluency
Post by: WWang1991 on March 11, 2015, 03:03:05 AM
Cell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time. Most of Cells should be passed when they are 90%-100% confluent.
Title: Re: Cell culture maintenance and confluency
Post by: TYuanyuan on June 15, 2015, 04:45:07 AM
In my experience is really not a problem if you passage them at 100%, especially with HEK cells. When you passage them just keep in mind to dilute them a bit more since you've more cells than usual.
Agree. it won't be that bad.