Methods and Techniques Discussion => Antigen Retrieval => Topic started by: NaNa on January 22, 2013, 07:05:00 AM

Title: antigen retrieval buffer
Post by: NaNa on January 22, 2013, 07:05:00 AM
Hi,
can I reuse the antigen retrieval buffer soln once I use it or I have to prepare it every immunostaining?
your help appreciated
Tnx.
Title: Re: antigen retrieval buffer
Post by: gula on January 22, 2013, 12:19:25 PM
If you use it boiling, you have to make it every time new. Water evaporates, ionstrength varies....
If you use it at temperatures about 70 degrees, I think, you can take it for more times.
gula
Title: Re: antigen retrieval buffer
Post by: NaNa on January 23, 2013, 05:12:40 AM
Thanks  ;)