General Research Topics => Molecular Biology => Topic started by: bufomarinus on October 30, 2004, 09:33:02 AM

Title: interference with DNA
Post by: bufomarinus on October 30, 2004, 09:33:02 AM
Hi everybody,

does anybody of you know if and how the following two immunoenzymatic staining procedures interfere with DNA and/or cause DNA damage:

1. BCIP/NBT Alkaline Phosphatase Substrate Solution
(5-bromo-4-chloro-3-indoyl phosphate and Nitroblue tetrazolium)

2. New Fuchsin Alkaline Phosphatase Substrate Solution
(New Fuchsin, Sodium Nitrite, Naphthol AS-Bis Phosphate, Levamisol)

Thanx,

Florian :wink:
Title: Re: interference with DNA
Post by: daryy on March 06, 2009, 08:40:51 PM
I make sure your questions are difficult to answer! I know nothing about DNA and DNA damage ;D
Title: Re: interference with DNA
Post by: ImmunoNYC on March 06, 2009, 11:35:25 PM
What you listed aren't procedures but are substrates. Are you asking about a whole procedure or those specific compounds? Either way we need more details.
Title: Re: interference with DNA
Post by: TYuanyuan on May 14, 2015, 04:46:14 AM
I have the same puzzle. Are you asking about a whole procedure or those specific compounds? More details are needed to anwer this question.
Title: Re: interference with DNA
Post by: VTimberlake on August 10, 2015, 09:51:01 PM
I guess http://www.creative-diagnostics.com/RNA-immunoprecipitation-RIP.html (http://www.creative-diagnostics.com/RNA-immunoprecipitation-RIP.html) might be helpful, good luck.