Antibody-Based Technologies => Antibodies Review => Antibody E => Topic started by: margarete on November 02, 2004, 10:54:01 AM

Title: er beta
Post by: margarete on November 02, 2004, 10:54:01 AM
I would greatly appreciate any advice (protocol) for ER-beta. :?
Thank you very much
Margaret
Title: Re: er beta
Post by: Spencer on January 06, 2010, 04:36:19 PM
Pa1-01 is the Ab we use.  Manufacturer suggest 1:50 dilution butI've found 1:200 works just as well. 
AR: 10mM sodium citrate pH 6.0 30 min at 96C
Quench: 5% h2o2 in Methanol 30 min
Block: 5% BSA 5% Nonfat milk 5% FBS 1 hour
1 ab 1:200 dilution o/n
2nd 1:500 anti rabbit 1 hour
ABC 30 min
DAB
Counterstain, dehydrate, coverslip done!