General Research Topics => General Discussion => Topic started by: SSpeese on December 03, 2013, 01:18:07 AM

Title: Staining for rRNA with the Y10B antibody
Post by: SSpeese on December 03, 2013, 01:18:07 AM
I am wondering if anyone has any experience using the Y10B antibody to stain for rRNA.  Supposedly, it recognizes the 5.8S rRNA.  The antibody is a mouse IgG3.  I have not had much luck getting results on whole mount Drosophila tissue that look like the results I have seen from cultured cells.  The main difference is that I get almost no signal from the nucleolous, which should have a high concentration of 5.8S rRNA.  The images from almost every cell culture image I have seen shows high levels of staining in the nucleolous.

Thanks,
   Sean Speese

Research Assistant Professor
OHSU
Title: Re: Staining for rRNA with the Y10B antibody
Post by: AnneHowell on April 15, 2015, 03:59:37 AM
I think it may be the quality problem of your antibody, where did you get it?.  It should be no problem to use rRNA Antibody (Y10b) .
Title: Re: Staining for rRNA with the Y10B antibody
Post by: JJoanna on June 27, 2016, 05:59:47 AM
I think it may be the quality problem of your antibody, where did you get it?.  It should be no problem to use rRNA Antibody (Y10b) .

mouse IgG3 antibody from http://goo.gl/y99sTZ