Methods and Techniques Discussion => Immunofluorescence (IF) => Topic started by: LNaranjo on October 13, 2014, 12:43:58 PM

Title: Autofluorescence in epithelial ovaric cancer
Post by: LNaranjo on October 13, 2014, 12:43:58 PM
Hi everyone,

i'm having problems of autofluorescence with this tissue :(

The biopsy is fixed with Bouin, the problem is that i just receive the slides done :-\ i'm pretty sure that the bouin is the reason of the autofluorescence.

someone have worked with this fixator in IF before?? can someone help me with a protocol?

Thanks  :)