General Research Topics => General Discussion => Topic started by: angela on January 12, 2015, 11:44:49 AM

Title: Is biotin blocking necessary on human spleen IHC?
Post by: angela on January 12, 2015, 11:44:49 AM
Hi everybody,

I have a question about biotin blocking. I have to do some IHC staining on human spleen paraffin sections. I remember that biotin blocking was quite important when performing IHC on mouse spleen, and I'm wondering how crucial this is in human spleen.

Thank you in advance for your comments,

Angela  :)
Title: Re: Is biotin blocking necessary on human spleen IHC?
Post by: gula on January 14, 2015, 09:41:39 AM
yes, you should block the endog. biotin.
gula
Title: Re: Is biotin blocking necessary on human spleen IHC?
Post by: immuno on March 05, 2015, 11:11:39 AM
Hi:

If you are using a biotin-based method you should block it, if you are using a polymer detection sistem like Envision this step is not necesary.

best

Immuno

Title: Re: Is biotin blocking necessary on human spleen IHC?
Post by: SBella on March 29, 2015, 01:52:12 AM
agreed immuno. I know biotin is quite important for human,  also called as vitamin H or coenzyme R; Biotin not only to prevents hair loss, it also plays an important role in maintaining the health of the skin. As for the effectiveness of the nervous system stability has not yet been confirmed, but it certainly has some help in the depression, insomnia.