Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: EBallarini on April 21, 2015, 11:58:13 AM

Title: CYP11A1 IHC-P
Post by: EBallarini on April 21, 2015, 11:58:13 AM
Can anyone suggest me a good Ab to stain CYP11A1 (cytochrome P45011a1) on mouse tissue?

Good morning.

I have a problem. I'm trying to stain CYP11A1 on Mouse tissues embedded in parrafin or frozen but I can't find a good Ab.

I tryed 3 different Ab on mouse testis and adrenal gland (positive control) embedded in paraffin or frozen in OCT . I changed many protocols (antigen retrival, blocking, Ab concentration, washing buffers, timing, etc etc) I wrote to all techical support and to authors of papers who used the same Ab but I never was able to obtain a good and specific staining (low signal and high background).

These are the Ab I used:

    Abcam cyp11a1 ab 75497
    Santa cruz cyp11a1 (D-16) sc18040
    Proteintech 133663-1-AP

Can you suggest a different Ab to use?
Title: Re: CYP11A1 IHC-P
Post by: WWang1991 on April 30, 2015, 03:35:49 AM
maybe this http://www.creative-bioarray.com/ (http://www.creative-bioarray.com/)can help you out
Title: Re: CYP11A1 IHC-P
Post by: EBallarini on April 30, 2015, 04:22:40 AM
Thank you very much!
I'll try!

Regards
Elisa
Title: Re: CYP11A1 IHC-P
Post by: YKonstantin on July 28, 2016, 05:51:12 AM
Hello,
I would recommend to search in an antibody database available at www.labome.com. For antibodies to CYP11A1 you may check this link: http://www.labome.com/review/gene/human/DRD1-antibody.html. In addition to the antibodies you have mentioned, Santa Cruz also has goat polyclonal (C-16) and rabbit polyclonal (H-165) antibodies suitable to detect CYP11A1 on mouse tissues. Also, OriGene has a rabbit polyclonal antibody to CYP11A1 suitable for IHC. In addition, there are several rabbit polyclonal antibody to CYP11A1, provided by Invitrogen (PA5-37359), MyBioSource (MBS128059) and US Biological (210368), which can be used for IHC on mouse tissues.
Title: Re: CYP11A1 IHC-P
Post by: MMaggie on October 03, 2016, 05:26:37 PM
thanks for the mention of CYP11A1 - (MBS128059) (https://www.mybiosource.com/prods/Antibody/Polyclonal/CYP11A1/datasheet.php?products_id=128059/) if you have any questions or concerns let me know and I'll be happy to answer them.