Methods and Techniques Discussion => Immunocytochemistry (ICC) => Topic started by: innsbrucker on January 20, 2018, 06:47:43 PM

Title: How to avoid salt crystallization in Immunocytochemistry?
Post by: innsbrucker on January 20, 2018, 06:47:43 PM
I did an ICC and while imaging, I found that there are salt crystals on the coverslips. How can I avoid it?

My last steps are as follows:
After secondary, I wash the coverslips with PBS 3 times for 5 min.

I put a drop of my mounting media on a glass slide.

Take out the coverslip from the PBS, gently tap it on a kim wipe and place it on the glass slide.

Next, I keep the slides overnight at RT in dark and the following day I seal them using nail varnish.

Thanks