Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: CSuzanne on February 13, 2018, 11:32:49 AM

Title: Fc Fusion proteins used as IHC primary antibody
Post by: CSuzanne on February 13, 2018, 11:32:49 AM
Hi guys,

I am reviewing data from a CRO attempting to develop an IHC assay for us using a fusion protein and trying to detect its receptor via a standard IHC frozen tissue method with brief acetone fixation.  I don't believe it is working and am trying to find some literature on using Fc fusion proteins in this manner to better understand what conditions one needs to use, sensitivity of the assay, etc.   Does anyone have experience with this?

Thanks, Suzanne