Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: DValerie on February 23, 2018, 01:45:07 PM

Title: Unspecific staining in skin
Post by: DValerie on February 23, 2018, 01:45:07 PM
Hi!

I am doing IHC in paraffin-embedded tissues and I have problems with a unspecific staining. I work with equine wounds and I know that unspecific staining has been seen in sebaceous glands, but I have one also in the blood clot. I made a test to check if this was probably caused by the endogen avidin/biotin and I got a positive staining even without primary and secondary antibodies (I use ABC with alkaline phosphatase). So, do you guys think I should block endogen avidin/biotin or this may due to something else?

Thank you!