General Research Topics => General Discussion => Topic started by: angela on April 12, 2018, 05:19:28 AM

Title: degrease or not degrease?
Post by: angela on April 12, 2018, 05:19:28 AM
Hi, I have a general question concerning degreasing of sections. I've been asked to stain some frozen sections from human brain, fixed and cryo-protected. I was supposed to use the same protocol as some other colleagues did, and according to that, I should also use a degreasing step with 100% acetone for 45' before starting the staining. The reason for this step would be that we would like to see some antigens that would be located under the myelin. But I'm wondering if that is really necessary. I'm also concerned about some of the stainings that I would have to do, since some are receptors and I'm afraid they could be washed off by the degreasing step.
Any suggestion is welcome.
Thanks. Angela