Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: Histolab on June 01, 2018, 09:09:32 AM

Title: Ph 6:00
Post by: Histolab on June 01, 2018, 09:09:32 AM
Hi,

I will be perfusing rodent brains using 4% PFA and PBS at PH 6:00, but I am concerned that for IEg staing for C-fos and EGR-1 (Zif) the incubation medium is always used at PH 7.4, is the preparation of the tissue at PH 6.0 going to interfere with the c-fos/Zif ractivity?

Any advice will be appreciated