Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: EAlberts on August 07, 2018, 10:00:41 AM

Title: Black flakes on slide staining
Post by: EAlberts on August 07, 2018, 10:00:41 AM
Hi I am fairly new to IHC,

I have done some staining recently which has come out quite nicely, but recently I have had some that come out with black flakes on the top - does anyone know what this might be? Is it DAB precipitate or contamination from contaminated PBS?

Thanks,

Ellie
Title: Re: Black flakes on slide staining
Post by: KRyan1800 on November 13, 2018, 02:22:56 PM
What tissue are you staining? I think I may have your exact problem with my small intestine tissue. I was told by a pathologist that you tend to see those black flakes with mucosal tissue like intestine or salivary glands because they are sticky. She said it was crystallized hematoxylin and that filtering it with a paper towel before should do the trick. I have not had that problem since.

Best,

Ryan