Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: NSabine on September 05, 2018, 05:10:30 PM

Title: Precipitation on slides when dehydrating
Post by: NSabine on September 05, 2018, 05:10:30 PM
Hello--

Hoping IHC experts can help with this dilemma:

When going from 70% ETOH --> 100% ETOH--> Citrosolv (citrus smelling xylene) there is progressive precipitation on the slide (milky waxy droplets/streaks esp when going fr 100%ETOH to Citrosolv). I've changed out all the ETOH and Xylene multiple times now.  Losing several slides and days of work each time this happens. 

Please help! Any input greatly appreciated.


Thanks!