Methods and Techniques Discussion => Immunohistochemistry (IHC) => Topic started by: RJason on October 02, 2018, 09:48:22 AM

Title: IHC artifact
Post by: RJason on October 02, 2018, 09:48:22 AM

We are having artifact on our IHC slides. I am hoping someone on this forum has encountered this and can give us some advice.

We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine.
We have tried a number of things but we still canít get consistent control of this issue.
Our rundown is standardized, we only use distilled water in our water baths, we take good care of our machine, clean the probe often, and refrigerate our red kits between runs.
Any ideas?
Title: Re: IHC artifact
Post by: ABerisha on October 17, 2018, 11:20:30 AM
hello RJason

Did you solve the problem with the AP artifact? because we have also the same issue with the Bond AP Kit.

Kind regards