Antibody-Based Technologies => Tumor Markers Review => Topic started by: merav fraenkel on February 13, 2005, 05:03:20 AM

Title: staining of psammomys pancreas with rabbit anti HNE
Post by: merav fraenkel on February 13, 2005, 05:03:20 AM
I followed the protocol published in this website to stain pancreatic sections of diabetic psammomys obesus without success. I tried overnight incubation at 4 degrees and also tried using a florescent secondary AB withut succuss either.
Does anyone have any further suggestions?
Merav Fraenkel
Title: staining of psammomys pancreas with rabbit anti HNE
Post by: excalibur on February 13, 2005, 08:01:25 AM
Are your sections frozens or paraffin?
Title: staining of psammomys pancreas with rabbit anti HNE
Post by: richard03 on February 13, 2005, 10:02:07 AM
The antibody may not have cross reactivity with human tissue.

Richard
Title: staining of psammomys pancreas with rabbit anti HNE
Post by: merav fraenkel on February 14, 2005, 12:51:00 AM
Quote from: "excalibur"
Are your sections frozens or paraffin?



They are paraffin embedded
Merav
Title: staining of psammomys pancreas with rabbit anti HNE
Post by: excalibur on February 14, 2005, 08:43:05 AM
You may need to do an antigen retrieval procedure such as boiling in citrate buffer to unmask your antigen.
Title: staining of psammomys pancreas with rabbit anti HNE
Post by: merav fraenkel on February 15, 2005, 01:36:04 AM
Quote from: "excalibur"
You may need to do an antigen retrieval procedure such as boiling in citrate buffer to unmask your antigen.


I did that already although this is not a nuclear Ag