Antibody-Based Technologies => Tumor Markers Review => Topic started by: pharmacomona on April 29, 2005, 05:08:12 PM

Title: antibodies to monitor wound healing
Post by: pharmacomona on April 29, 2005, 05:08:12 PM
I would like to know if anyone has experience with monitoring healing in rat skin as a model of acute wounds. I am currently doing work in this area and would like to know the "best" growth factors and cytokines to monitor with regards to inflammation, granulation tissue formation and remodelling. By "best" i refer to expression of the growth factors in the skin. My previous attempts using IL1 beta faltered becuase it is not highly expressed. I also tried CD105 as an angiogenic marker but i could only find companies that makes it for the mouse giving me less than 85% epitope homology between the specie.

Post-grad student in jamaica...help!
Title: antibodies to monitor wound healing
Post by: richard03 on May 02, 2005, 12:06:15 AM
You may try growth factors such as TGF-alpha, TGF-beta, EGF, IGF-1, etc. For angio markers, you may try VEGF. The following link may help.

http://herkules.oulu.fi/isbn9514271106/html/x845.html

http://www.bme.utexas.edu/faculty/schmidt/Courses/BME379/TeamPresentations/WoundFinal.ppt

Richard