Antibody-Based Technologies => Antibodies Review => Antibody B => Topic started by: bn on June 20, 2005, 01:55:38 PM

Title: Beta-galactosidase mononclonal
Post by: bn on June 20, 2005, 01:55:38 PM
Does anyone know of a monoclonal antibody to Beta-galactosidase that works for formaldehyde fixed, paraffin embedded tissue? I want to detect beta-gal in embryonic mouse sections and although I have a good rabbit polyclonal to beta-gal, I need a mouse monoclonal so I can do double labelling with other rabbit antibodies. Alternatively, does anyone have a protocol for double immunofluorescence with two rabbit primary antibodies?
Many thanks in advance.
Title: Beta-galactosidase mononclonal
Post by: richard03 on June 22, 2005, 08:33:04 PM
If you use mouse monoclonal on mouse tissue, it will produce heavy background, especially for embryonic tissues.

I am confused. You are looking for monoclonal beta-gal and double label with rabbit b-gal?

If you use two primary from same species, you should do sequential method, meaning one after another.

Here is a protocol for beta-gal staining

http://www.ihcworld.com/_protocols/antibody_protocols/b-gal_abcam.htm

Richard