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Immunohistochemistry (IHC) / CD11b on rabbit lung.
« Last post by AWagenaar on August 02, 2019, 04:07:01 AM »

I did a cd11b staining on rabbit lung but i get different results.
from not colored to a nice coloring.
I used the same protocol everytime. and it's also in the same Anti-body LOT#
General Histology / Acridine Orange Protocol
« Last post by microoz on April 23, 2019, 04:28:00 AM »

I am looking for an acridine orange staining protocol for paraffin embedded sections. There is quite a lot out there for staining cells, but precious little for staining paraffin embedded sections.

Any help will be very much appreciated. Thank you.

Apoptosis or Cell Death / Re: detection of apoptosis
« Last post by PDavies83 on January 04, 2019, 06:27:53 AM »
Many apoptosis ELISA kits are available which can help in learning how cells are undergoing suicide in a predictable, programmed, controlled and routine manner. You may find the below link helpful.

Tumor Markers Review / Re: Tumor Cells
« Last post by PDavies83 on January 04, 2019, 06:23:00 AM »
There are many specific tumor marker ELISA kits that are available which can be used to detect, diagnose and manage many different types of cancers. At present, a large number of tumor markers have already been extensively characterised and are being widely utilised in clinical applications. You may find the below link helpful.

Tumor Markers Review / Re: Tumor Markers Review
« Last post by PDavies83 on January 04, 2019, 06:01:06 AM »
Many tumor markers play a critical role in different clinical applications and these biomarkers have also been extensively characterised. Some of these are known to be linked to only one specific type of cancer whereas other can be associated with many types of cancers. Currently, there is no universal tumor maker which can be used to detect any type of cancer but instead specific markers need to be used for specific cancers. You may find the below link useful, since it listís many tumor markers and which cancers each one is associated with.
Recently, scientists from the Dana-Farber Cancer Institute have discovered new drug targets for two malignant cancers. It is expected to develop new therapies for the treatment of synovial sarcoma and malignant rods-shaped tumors. Related research result was published in the Nature Cell Biology.


The researchers say that these two cancers depend on a new molecular called ncBAF, which plays a key role in regulating gene activity, consisting of multiple specific protein subunits. Biologically or chemically inactivating components of ncBAF may specifically impair proliferation of synovial sarcoma and malignant rod-shaped tumor cancer cell lines. Dr. Cigall Kadoch said that this is an important research result they have achieved in the treatment of stubborn invasive cancer. In that study, they have identified a new type of cancer-specific target, which is expected to be used in the future to develop new anticancer therapies.

continue to read: https://www.creativebiomart.net/blog/new-drug-targets-that-are-expected-to-treat-malignant-cancer-discovered/
Neuroscience / Re: Somatostatin IHC help
« Last post by RSabine18 on December 27, 2018, 10:46:25 AM »
This is a late answer, but...

We are currently using a protocol to visualise somatostatin with immunofluorescence on 50 Ķm-thick free-floating mouse brain sections, and it works fairly well.
Primary antibody : polyclonal, made in goat, D-20 from Clinisciences, used 1/500 (overnight at 4įC)
Secondary antibody  : Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488, used 1/1000 (3h at room temperature).
-   Washing buffer : PBS + 1% Normal Donkey Serum + 1% gelatin from cold water fish skin
-   Blocking Solution : PBS + 5%  Normal Donkey Serum + 2% gelatin from cold water fish skin + 0.2% TritonX-100
-   Diluting buffer for antibodies : PBS + 1% Normal Donkey Serum+ 1% gelatin from cold water fish skin + 1% DMSO + 0.2% TritonX-100

I hope it may help you.
Good luck,

Immunohistochemistry (IHC) / Re: Black flakes on slide staining
« Last post by KRyan1800 on November 13, 2018, 02:22:56 PM »
What tissue are you staining? I think I may have your exact problem with my small intestine tissue. I was told by a pathologist that you tend to see those black flakes with mucosal tissue like intestine or salivary glands because they are sticky. She said it was crystallized hematoxylin and that filtering it with a paper towel before should do the trick. I have not had that problem since.


Immunohistochemistry (IHC) / Re: IHC artifact
« Last post by ABerisha on October 17, 2018, 11:20:30 AM »
hello RJason

Did you solve the problem with the AP artifact? because we have also the same issue with the Bond AP Kit.

Kind regards
Immunohistochemistry (IHC) / IHC artifact
« Last post by RJason on October 02, 2018, 09:48:22 AM »

We are having artifact on our IHC slides. I am hoping someone on this forum has encountered this and can give us some advice.

We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine.
We have tried a number of things but we still canít get consistent control of this issue.
Our rundown is standardized, we only use distilled water in our water baths, we take good care of our machine, clean the probe often, and refrigerate our red kits between runs.
Any ideas?

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