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1
Apoptosis or Cell Death / Re: detection of apoptosis
« Last post by PDavies83 on January 04, 2019, 06:27:53 AM »
Many apoptosis ELISA kits are available which can help in learning how cells are undergoing suicide in a predictable, programmed, controlled and routine manner. You may find the below link helpful.

http://www.elisakits.co.uk/immunology-cytokines/apoptosis/
2
Tumor Markers Review / Re: Tumor Cells
« Last post by PDavies83 on January 04, 2019, 06:23:00 AM »
There are many specific tumor marker ELISA kits that are available which can be used to detect, diagnose and manage many different types of cancers. At present, a large number of tumor markers have already been extensively characterised and are being widely utilised in clinical applications. You may find the below link helpful.

http://www.elisakits.co.uk/category/tumor-markers/
3
Tumor Markers Review / Re: Tumor Markers Review
« Last post by PDavies83 on January 04, 2019, 06:01:06 AM »
Many tumor markers play a critical role in different clinical applications and these biomarkers have also been extensively characterised. Some of these are known to be linked to only one specific type of cancer whereas other can be associated with many types of cancers. Currently, there is no universal tumor maker which can be used to detect any type of cancer but instead specific markers need to be used for specific cancers. You may find the below link useful, since it listís many tumor markers and which cancers each one is associated with.
 
http://www.elisakits.co.uk/tumor-markers/
4
Recently, scientists from the Dana-Farber Cancer Institute have discovered new drug targets for two malignant cancers. It is expected to develop new therapies for the treatment of synovial sarcoma and malignant rods-shaped tumors. Related research result was published in the Nature Cell Biology.

 

The researchers say that these two cancers depend on a new molecular called ncBAF, which plays a key role in regulating gene activity, consisting of multiple specific protein subunits. Biologically or chemically inactivating components of ncBAF may specifically impair proliferation of synovial sarcoma and malignant rod-shaped tumor cancer cell lines. Dr. Cigall Kadoch said that this is an important research result they have achieved in the treatment of stubborn invasive cancer. In that study, they have identified a new type of cancer-specific target, which is expected to be used in the future to develop new anticancer therapies.

continue to read: https://www.creativebiomart.net/blog/new-drug-targets-that-are-expected-to-treat-malignant-cancer-discovered/
5
Neuroscience / Re: Somatostatin IHC help
« Last post by RSabine18 on December 27, 2018, 10:46:25 AM »
This is a late answer, but...

We are currently using a protocol to visualise somatostatin with immunofluorescence on 50 Ķm-thick free-floating mouse brain sections, and it works fairly well.
Primary antibody : polyclonal, made in goat, D-20 from Clinisciences, used 1/500 (overnight at 4įC)
Secondary antibody  : Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488, used 1/1000 (3h at room temperature).
-   Washing buffer : PBS + 1% Normal Donkey Serum + 1% gelatin from cold water fish skin
-   Blocking Solution : PBS + 5%  Normal Donkey Serum + 2% gelatin from cold water fish skin + 0.2% TritonX-100
-   Diluting buffer for antibodies : PBS + 1% Normal Donkey Serum+ 1% gelatin from cold water fish skin + 1% DMSO + 0.2% TritonX-100

I hope it may help you.
Good luck,

Sabine
6
Immunohistochemistry (IHC) / Re: Black flakes on slide staining
« Last post by KRyan1800 on November 13, 2018, 02:22:56 PM »
What tissue are you staining? I think I may have your exact problem with my small intestine tissue. I was told by a pathologist that you tend to see those black flakes with mucosal tissue like intestine or salivary glands because they are sticky. She said it was crystallized hematoxylin and that filtering it with a paper towel before should do the trick. I have not had that problem since.

Best,

Ryan
7
Immunohistochemistry (IHC) / Re: IHC artifact
« Last post by ABerisha on October 17, 2018, 11:20:30 AM »
hello RJason

Did you solve the problem with the AP artifact? because we have also the same issue with the Bond AP Kit.

Kind regards
8
Immunohistochemistry (IHC) / IHC artifact
« Last post by RJason on October 02, 2018, 09:48:22 AM »
Hello,

We are having artifact on our IHC slides. I am hoping someone on this forum has encountered this and can give us some advice.

We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine.
We have tried a number of things but we still canít get consistent control of this issue.
Our rundown is standardized, we only use distilled water in our water baths, we take good care of our machine, clean the probe often, and refrigerate our red kits between runs.
Any ideas?

https://drive.google.com/open?id=1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA
9
Immunohistochemistry (IHC) / Precipitation on slides when dehydrating
« Last post by NSabine on September 05, 2018, 05:10:30 PM »
Hello--

Hoping IHC experts can help with this dilemma:

When going from 70% ETOH --> 100% ETOH--> Citrosolv (citrus smelling xylene) there is progressive precipitation on the slide (milky waxy droplets/streaks esp when going fr 100%ETOH to Citrosolv). I've changed out all the ETOH and Xylene multiple times now.  Losing several slides and days of work each time this happens. 

Please help! Any input greatly appreciated.


Thanks!
10
Immunohistochemistry (IHC) / Tissue shredded after peroxidase IHC
« Last post by MAmanda on August 22, 2018, 03:50:37 PM »
Quick question. Why would brain slices be shredded after peroxidase IHC?

Two sets of tissue, both perfused (4%PFA) by different people. Both sets sectioned on cryostat by different people. Kept in antifreeze.
Both ran through peroxidase IHC in the exact same manner.
TBS (1x) washes, methanolic peroxide wash (15 min), TBS+ blocking wash. Overnight Primary antibody. Then washes, secondary, tertiary streptavadin incubation. DAB for blue-black reactivity.

One set of tissue was "shredded" at the end of the IHC. the other remained intact remarkably well. Trying to find out what could be compromising the structural integrity of the tissue either during perfusion or sectioning on the cryostat, as those are the only 2 times the tissue were treated differently. Thank you in advance for any comments
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