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91
Streptavidin—signal amplification for imaging.
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Neuroscience / Re: Experimental question
« Last post by JJoanna on June 27, 2016, 03:14:44 AM »
Why not use protein carbonyl kit? It's more accurate and convenient.
Protein Carbonyl Protocol from Mouse Metabolic Phenotyping Centers:
https://mmpc.org/shared/document.aspx?id=123&doctype=Protocol
Protein Carbonyl Assays and Reagents from Cell Biolabs, Inc.:
http://www.cellbiolabs.com/protein-carbonyl-assays-and-reagents?gclid=CNbBuufSx80CFQp_vQodxIABSw
Protein Carbonyl Assay Kit from Creative Proteomics:
http://www.creative-proteomics.com/cptk0573-product-protein-carbonyl-assay-kit-39490.htm
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Miscellaneous / IHC question
« Last post by KWinslet on June 26, 2016, 11:57:38 PM »
IHC question
I wish to do IHC on rat tissue for confocal microscopy that I would be using flourescent tagged antibodies from creative diagnostics to colocalize 3 molecules in a single slide section. So Can I use polyclonal antibodies?
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Miscellaneous / Innate and Adaptive Immunity
« Last post by KWinslet on June 26, 2016, 11:50:43 PM »
Defense against microbes is mediated by the early reactions of innate immunity and the later responses of adaptive immunity. (Figure 1, 2; Table 1)
Innate immunity (also called natural or native immunity) provides the early line of defense against microbes. It consists of cellular and biochemical defense mechanisms that are in place even before infection and are poised to respond rapidly to infections. The mechanisms of innate immunity are specific for structures that are common to groups of related microbes and may not distinguish fine differences between microbes. The principal components of innate immunity are:
(1) Physical and chemical barriers, such as epithelia and antimicrobial chemicals produced at epithelial surfaces;
(2) Phagocytic cells (neutrophils, macrophages), dendritic cells, and natural killer (NK) cells and other innate lymphoid cells;
(3) Blood proteins, including members of the complement system and other mediators of inflammation.
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Miscellaneous / Pharmacokinetics Elisa
« Last post by KWinslet on June 24, 2016, 04:25:26 AM »
Pharmacokinetics, abbreviated as PK, is a branch of pharmacology dedicated to determining the fate of substances administered externally to a living organism. The substances of interest include pharmaceutical agents, hormones, nutrients, and toxins. Pharmacokinetics ELISA Kits for the quantitation of serum protein drug levels to provide accurate pharmacokinetic (PK) data that will guide the optimization of drug dosing regimens.
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Antibody E / Re: Elastin antibody
« Last post by JJoanna on June 24, 2016, 04:22:46 AM »
Anti-ELN monoclonal antibody, clone 21C9 (DCABH-9817)
Anti-ELN monoclonal antibody, clone CB5 (DCABH-8109)
Anti-ELN monoclonal antibody, clone CB-5 (DMAB5394MH)

link:http://goo.gl/mCv38W

Hope they can help you!
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Western Blotting (WB) / Re: mistake
« Last post by JJoanna on June 24, 2016, 04:11:16 AM »
Pre-cast should be stored in refrigerator (2–8°C). Do not freeze. You'd better use a new one.
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Pharmacokinetics ELISA kit for the quantitation of serum protein drug levels to provide accurate pharmacokinetic (PK) data that will guide the optimization of drug dosing regimens.Anti- Rituximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Rituximab present in biological matrices is bound by the immobilized anti- Rituximab antibody. After washing away any unbound substances, enzyme linked anti- Rituximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Rituxan (domain residing in Fc portion of the Rituxan molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Rituximab present in test samples. The color development is stopped and the intensity of the color is measured.
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General Discussion / Re: Co-Immunoprecipitation
« Last post by KWinslet on June 24, 2016, 03:34:38 AM »
If you are looking for the co-ip product. First do the lysis in following buffer (25 mM Tris-HCl pH 7.4 or pH8.0 + 100 mM NaCl+50 mM NaF+ 2mM EDTA+ Protease inhibitor + 0.5% Tx100).  If protein is soluble then Tx100 is very good but in case of  Membrane protein you may have to use 2mM DDM instead to Tx100. Lysis should be done at 4 degree and clear the insoluble fraction by centrifugation  at 2000g/15 min.  Do the co-ip with ab/beads for 1 hr (maximum 90 min). Wash the beads 4x with same lysis buffer and keep the centrifugation speed same.

Elute the protein by adding 50 ul beads (for 1ug antibody per IP) for 95 degree 3 min.
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General Discussion / Re: Recombinant Human Activin A
« Last post by JJoanna on June 23, 2016, 04:26:27 AM »
Activin A is a member of the transforming growth factor β (TGF-β) superfamily. It has a wide range of biological activities including driving endoderm and mesoderm induction and regulating the differentiation of many cell types. Activin A has also been shown to support maintenance of human embryonic stem cells in the undifferentiated state. It shares receptors with the ligand nodal and activates the same downstream SMAD pathway as TGF-β. Recombinant human mature Activin A is a disulfide-linked homodimer of two 116 amino acid residue βA subunits with a molecular weight of 13 kDa.

Human Activin A expressed in CHO cells or Baculovirus-Insect Cells or Nicotiana benthamiana.

Purity:Greater than 95% as determined by SDS-PAGE and visualized by silver stain.

From:goo.gl/VUm2cq
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