Author Topic: good + control for TUNEL-Enzyme method  (Read 6783 times)

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Offline Jennybean

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good + control for TUNEL-Enzyme method
« on: August 03, 2005, 01:50:29 PM »
Hello- I'm an IHC newbie and I'd like to test for apoptosis in sections of mouse mammary and testicluar tumors (originally fixed in NBF) using the TUNEL-enzyme method. I'd like to buy slides to use as positive controls as opposed to making them myself. Any good suggestions?

good + control for TUNEL-Enzyme method
« on: August 03, 2005, 01:50:29 PM »

Offline richard03

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good + control for TUNEL-Enzyme method
« Reply #1 on: August 03, 2005, 11:51:00 PM »
Chemicon sell positive control slides come with apoptosis kits. I am not sure if they sell slides separately? You give it a try.

http://www.chemicon.com/Product/ProductDataSheet.asp?ProductItem=S7115

Offline ImmunoNYC

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Re: good + control for TUNEL-Enzyme method
« Reply #2 on: November 25, 2005, 12:11:18 AM »
I think using a homemade DNase treated slide is the way to go. I do it all the time, it's easy and a good control (and cheap!).

Re: good + control for TUNEL-Enzyme method
« Reply #2 on: November 25, 2005, 12:11:18 AM »