Antibody-Based Technologies > Antibody E

ER/PR on archive material

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madeleine huey:
Hi,
ER/PR are not recommend baking too long (bake no longer than 1 hr @ 60C).  Here's my experience for these 2 markers.  1) Bake paraffin section only same day you use  2) Never bake more than once (some laboratory still practice baking for than once after cutting, and leaveing sections in RT for months & years).  
You might want to re-cut some fresh section & try it with the old ab.  I beg you will get the stain this time.
Let me know if they don't work.
Madeleine

ole:
Hi Phil
im currently reading thru my old articles, found an article that "touches" the issue.
Even though the article is about reactivity of stored paraffin slides, they compare with recut slides (old blocks), .... some interesting thoughts about storage etc. Perhaps you could store a few blocks cold a few years and compare with some stored "hot" (your warm climate).

in case you have not read it........

Bertheau P, ........
Variability of immunohistochemical reactivity on stored paraffin slides.
J Clin Pathol 1998; 51: 370-374.

I have a good article somewhere about "what affects ER activity", it doesnt mention storage PET as an factor,  but ill find it.

Ole

ole:
Phil
Was not quite what thought i remembered "the article"  :oops: but still........

Daniel A Arber.
Effect of Prolonged Formalin fixation on the immunohistochemical Reactivity of Breast Markers
Applied immunohistochemical and mol morp 10(2): 183-186, 2002

If you have tried it?
I find the SP1 antibody to work best with AR (superheating in TE pH 9), even though both articles about the "novell" antibodies and perhaps datasheet claims that it works optimal also without AR.

Ole

philtrougouboff:
Thanks a lot for the references.I'll have a look soon.
Phil  :D

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