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Author Topic: Basic TEM questions  (Read 5994 times)

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Offline Tony

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Basic TEM questions
« on: July 02, 2003, 10:28:01 PM »
I am about to start TEM project and currently learning this technique. A few questions to ask.

1. How big the block should be appropriate size?
2. Osmium for 1 hour. Can I fix overnight?
3. After embedding in capsal, how long should the block incubated in 65 C oven, 24 hour or 48 hour?
4. Do I have to do thick sections before thin sections?

Any help is appreciated.

Tony

Basic TEM questions
« on: July 02, 2003, 10:28:01 PM »

davidtest

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Basic TEM questions
« Reply #1 on: July 05, 2003, 03:25:16 PM »
1. The size of blocks should be around 1 cubic mm
2. No. You can only fix tissue with osmium for 1-2 hours long and no longer.
3. Baking of embedded blocks should be at least 24 hours. I have used 72 hours incubation time without any noticeable problem.
4. You don't need to do thick section if the area of precision is not important to your study.

David

Basic TEM questions
« Reply #1 on: July 05, 2003, 03:25:16 PM »