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Author Topic: For help! antigen retrieval protocols  (Read 4764 times)

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Offline daiyan1828

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For help! antigen retrieval protocols
« on: September 22, 2005, 09:08:21 PM »
Please tell me the protocols on some antigen retrieval solutions ,such as  EDTA-Tris,Tris etc. when using pressure cooker ,how long you keep it?whether the low or high  temperature affects the antigen impression?

daiyan1828@yahoo.com.cn

For help! antigen retrieval protocols
« on: September 22, 2005, 09:08:21 PM »

Offline richard03

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For help! antigen retrieval protocols
« Reply #1 on: September 23, 2005, 09:16:26 AM »
Use the following page for all antigen retrieval protocols:

http://www.ihcworld.com/epitope%20retrieval.htm

Higher temp usually gives stronger staining intensity.

Richard

For help! antigen retrieval protocols
« Reply #1 on: September 23, 2005, 09:16:26 AM »