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Author Topic: Anti-mouse PCNA  (Read 9564 times)

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Offline Jennybean

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Anti-mouse PCNA
« on: September 23, 2005, 11:02:50 AM »
Hello...I didn't see a protocol for anti-mouse PCNA on the IHC World protocols list and I was wondering if anyone had a decent one or could at least recommend a good antibody. Please let me know. Thanks...Jen.

Anti-mouse PCNA
« on: September 23, 2005, 11:02:50 AM »

Offline richard03

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Anti-mouse PCNA
« Reply #1 on: September 23, 2005, 11:12:29 AM »
Santa Cruz Anti-PCNA sc-56 works will with mouse tissue. You can use the following protocol:

http://www.ihcworld.com/_protocols/antibody_protocols/pcna_santa_cruz.htm

However, if background is high, you need to use MOM blocking (some tissues do not need this blocking).

Richard

Offline broiler25

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Anti-mouse PCNA
« Reply #2 on: September 23, 2005, 12:06:57 PM »
Is there a specific reason you want to use PCNA?  Rabbit Monoclonal Ki67 works very well on mouse tissue and is a better marker of proliferation.

Offline ImmunoNYC

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Anti-mouse PCNA
« Reply #3 on: September 24, 2005, 12:10:57 PM »
Quote from: "broiler25"
Is there a specific reason you want to use PCNA?  Rabbit Monoclonal Ki67 works very well on mouse tissue and is a better marker of proliferation.

I completely agree. If you are looking for a proliferation marker, PCNA isn't the way to go. Ki67 is are far superior and there are other even more specific ones than Ki67 depending on what stage of the cell cycle you are interested in.

Offline Habbe

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Re: Anti-mouse PCNA
« Reply #4 on: October 01, 2007, 04:12:22 PM »
Forget PCNA. I have to agree to all otehrs- KI67 is the way to go!

Offline richard03

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Re: Anti-mouse PCNA
« Reply #5 on: October 01, 2007, 08:25:20 PM »
« Last Edit: January 15, 2008, 07:31:34 PM by richard03 »

Offline richard03

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Re: Anti-mouse PCNA
« Reply #6 on: October 01, 2007, 08:29:36 PM »
The best Ki67 antibody I have worked with is the one from Lab Vision - Rabbit Monoclonal antibody.

http://www.ihcworld.com/_protocols/antibody_protocols/ki67-sp6_labvision.htm

Offline marina77

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Re: Anti-mouse PCNA
« Reply #7 on: January 15, 2008, 12:26:30 PM »
I am surprised by all comments:))
Cause recently I have read a publication comparing Ki-67 and PCNA: It has been shown, that PCNA detect all proliferating cells, in contrast to Ki-67, wich does not recognize a part of them.

Re: Anti-mouse PCNA
« Reply #7 on: January 15, 2008, 12:26:30 PM »