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Author Topic: VEGF, VWF and PECAM  (Read 6940 times)

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Offline Leonard Cheung

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VEGF, VWF and PECAM
« on: November 25, 2005, 05:15:24 AM »
Which kind of antigen retrievals should be used in VEGF, VWF and PECAM stainning? Thanks!

VEGF, VWF and PECAM
« on: November 25, 2005, 05:15:24 AM »

Offline ImmunoNYC

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Re: VEGF, VWF and PECAM
« Reply #1 on: November 25, 2005, 10:34:14 AM »
Which clones or antibodies specifically - it makes a difference? What tissue - human or mouse?

Offline Leonard Cheung

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VEGF, VWF and PECAM
« Reply #2 on: November 25, 2005, 11:22:30 AM »
mouse VEGF, rabbit VWF and PECAM for SD rat skeletal muscle

Offline ImmunoNYC

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VEGF, VWF and PECAM
« Reply #3 on: November 25, 2005, 11:51:14 AM »
Which antibodies specifically? Sources, clones might help us to be more precise in our answers. It does make a difference - for example one clone of PECAM we have for mouse tissue works with different Ag retrieval than another we have!

Quote from: "Leonard Cheung"
mouse VEGF, rabbit VWF and PECAM for SD rat skeletal muscle

Offline Leonard Cheung

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VEGF, VWF and PECAM
« Reply #4 on: November 25, 2005, 12:49:10 PM »
Sorry...I dun understand what mean of sources and clones

Offline ImmunoNYC

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VEGF, VWF and PECAM
« Reply #5 on: November 25, 2005, 06:09:32 PM »
I mean, where did you buy the antibodies and what clones or preps are they? A goat anti-mouse CD31 might need a different antigen retrieval than a rat anti-mouse CD31.

Offline Leonard Cheung

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VEGF, VWF and PECAM
« Reply #6 on: November 25, 2005, 10:40:38 PM »
ic....the antibodies are the products of Santa Cruz, they are mouse anti-rat VEGF, rabbit anti-rat VWF and PECAM

VEGF, VWF and PECAM
« Reply #6 on: November 25, 2005, 10:40:38 PM »