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Author Topic: a new problem  (Read 2925 times)

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Offline danielfailde

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a new problem
« on: November 28, 2005, 12:36:31 PM »
Hello at all:
can you explain the Carr-Price technique to stain?
Thank you for the atention and your colaboration

a new problem
« on: November 28, 2005, 12:36:31 PM »

Offline excalibur

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a new problem
« Reply #1 on: November 28, 2005, 02:30:05 PM »
I am not familiar with this method. What tissue component does it stain? Maybe I can suggest some other method.

There is a procedure named only Carr's to demonstrate calcium using chloranilic acid.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline ImmunoNYC

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a new problem
« Reply #2 on: November 30, 2005, 11:18:48 PM »
I also am not familiar with the method but I googled it and what I found is that it is a colorimetric method to determine the content of carotenoids and vitamin A!? Interesting, but I am still not sure HOW it is used for histological purposes.

Offline excalibur

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a new problem
« Reply #3 on: December 01, 2005, 10:01:30 AM »
Hi Maximina, I also googled it and daniel is trying to stain some cells in a fish that contain carotenoids.
I suggested he fix the tissue, cut frozens sections, run them through water, alcohols, then to chloroform. (Xylene dissolves the carotenoids) Stain in a saturated solution of SbCl3 in chloroform for 5 or more minutes and his cells should stain blue.

Awaiting his results.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline ImmunoNYC

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a new problem
« Reply #4 on: December 01, 2005, 12:41:45 PM »
That is really cool. Well done! I also look forward to his results.

Quote from: "excalibur"
Hi Maximina, I also googled it and daniel is trying to stain some cells in a fish that contain carotenoids.
I suggested he fix the tissue, cut frozens sections, run them through water, alcohols, then to chloroform. (Xylene dissolves the carotenoids) Stain in a saturated solution of SbCl3 in chloroform for 5 or more minutes and his cells should stain blue.

Awaiting his results.

a new problem
« Reply #4 on: December 01, 2005, 12:41:45 PM »