Well, your procedure sounds fine. Just make sure your stains are fresh and maybe increase the times a little. Also try a 2% PFA/2% glut in a different buffer, such as cacodylate.
Remember also you are working with scar tissue which is alot of collagen, with fibroblasts few and far between. Did you do thick sections (1 micron) stained with Toluidine Blue to see what is in your block and you are where you want to be? Since these are tiny blocks, you may want to embed several, do thick sections, and then thin section and stain the ones containing the components you need.
Good luck! :wink: