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Author Topic: ki 67  (Read 4706 times)

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Offline Leonard Cheung

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ki 67
« on: December 07, 2005, 05:24:15 AM »
Can ki 67 be treated by proteinase K as antigen retrieval for mouse skeletal muscle (formalin, paraffin)? if yes, any conditions need? Thanks

ki 67
« on: December 07, 2005, 05:24:15 AM »

Offline richard03

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ki 67
« Reply #1 on: December 07, 2005, 07:22:07 AM »
Ki-67 is the best with HIER such as citrate pH6, or edta pH8. Enzyme digestion (PIER) will not work as good as HIER for this particular antibody.  Also digestion usually gives more tissue damage so I also try to avoid this if other retrieval methods worked.

Richard

Offline ImmunoNYC

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ki 67
« Reply #2 on: December 07, 2005, 10:00:15 PM »
I couldn't agree more Richard.

Quote from: "richard03"
Ki-67 is the best with HIER such as citrate pH6, or edta pH8. Enzyme digestion (PIER) will not work as good as HIER for this particular antibody.  Also digestion usually gives more tissue damage so I also try to avoid this if other retrieval methods worked.

Richard

ki 67
« Reply #2 on: December 07, 2005, 10:00:15 PM »