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Author Topic: problems in antigen retrieval  (Read 5103 times)

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Offline apple

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problems in antigen retrieval
« on: December 19, 2005, 08:16:18 PM »
Hi,

I tried high temperature antigen retrieval (using high Ph buffer in water bath) on my FFPE slides. I found this treatment could damage tissue, for example, cause holes inside tissue, cause tissue overlap etc. Has anyone have the same experience? How to avoid these problem?

Many thanks

Apple

problems in antigen retrieval
« on: December 19, 2005, 08:16:18 PM »

Offline richard03

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problems in antigen retrieval
« Reply #1 on: December 20, 2005, 04:50:03 PM »
You are right. High pH AR buffers tend to damage tissue more than that with lower pH. If you have to use high pH AR buffer, try shorter time and use the best adhesive slides (charged or coated sldies).

Richard

Offline apple

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problems in antigen retrieval
« Reply #2 on: December 20, 2005, 06:44:37 PM »
Thanks Richard.

problems in antigen retrieval
« Reply #2 on: December 20, 2005, 06:44:37 PM »